e381 - Peroxysome proliferator-activated receptor gamma agonist 15-deoxy-delta 12,14 prostaglandin J2 suppresses murine model of experimental autoimmune uveitis
 
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Topics
Retina - Animal studies - Immunology
Additional information
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Session information

This abstract has been assigned to session POSTERS: physiology, pharmacology, glaucoma, immunology.

This poster session will be presented on Saturday 11 October 2003.

 
Authors
Author 1CABAY LC
Departement of Ophthalmolgy, University of Aberdeen (Aberdeen)
Departement of Ophthalmolgy, CHU Saint-Pierre (Brussels)
I.R.I.B.H.N, Campus Erasme (Brussels)
Author 2WILLERMAIN WF
Departement of Ophthalmolgy, CHU Saint-Pierre (Brussels)
I.R.I.B.H.N, Campus Erasme (Brussels)
Author 3CASPERS-VELU LCV
Departement of Ophthalmolgy, CHU Saint-Pierre (Brussels)
Author 4BRUYNS CB
I.R.I.B.H.N, Campus Erasme (Brussels)
Author 5FORRESTER JV
Departement of Ophthalmolgy, University of Aberdeen (Aberdeen)
Abstract
Purpose
Peroxysome proliferator-activated receptor (PPAR) gamma agonist 15-deoxy-delta 12,14 prostaglandin J2 (15PGJ2) suppresses immune responses in previous studies. Here, we investigate effects of 15PGJ 2 in murine model of experimental autoimmune uveitis (EAU).
Methods
EAU was induced in B10RIII mice using IRBP 160-181 emulsified in Freund's complete adjuvant (CFA). Control mice received PBS and CFA. At day 15, draining lymph nodes and spleens were harvested for single-cell suspensions. Cells were cultured with 15PGJ2. Con-A and IRBP were used as stimulating agents. Proliferation assays were performed after 72 hours incubation ([H3] thymidine uptake assay). IL-10, IL-12 and IFN gamma were measured in supernatants by ELISA. Expression of CD69 and CD25 was assessed by flow cytometry.
Results
15PGJ2: (a) inhibited proliferation of Con-A activated splenocytes and lymph node cells obtained from normal mice in a dose dependant manner. (b) suppressed proliferation of IRBP activated cells from immunized mice (c) reduced IFN gamma and IL-12 secretion and increased IL-10 secretion, and (d) 15PGJ2 down-regulated CD25 and CD69 expression.
Conclusions
Our results confirm anti-inflammatory properties of 15PGJ2. In addition, we show that 15PGJ2 down-regulates immune cell activation in EAU in vitro. Our data, together with other immunosuppressive properties of 15PGJ2 on RPE cells, suggest that 15PGJ 2 may be a useful candidate for control of intraocular inflammation.